Overview

Product Description	Rabbit Polyclonal antibody recognizes Drebrin
Tested Reactivity	Hu, Ms, Rat
Tested Application	ICC/IF, IP, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	Drebrin
Antigen Species	Human
Immunogen	Synthetic peptide of Human Drebrin.
Conjugation	Un-conjugated
Alternate Names	Developmentally-regulated brain protein; D0S117E; Drebrin

Application Instructions

Application Suggestion	Tested Application Dilution ICC/IF 1:50 - 1:200 IP 1:50 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	HeLa
Observed Size	~ 125 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 1627 Human DBN1 GeneID: 56320 Mouse DBN1 GeneID: 81653 Rat DBN1 Swiss-port # Q07266 Rat Drebrin Swiss-port # Q16643 Human Drebrin Swiss-port # Q9QXS6 Mouse Drebrin More
Gene Symbol	DBN1
Gene Full Name	drebrin 1
Background	The protein encoded by this gene is a cytoplasmic actin-binding protein thought to play a role in the process of neuronal growth. It is a member of the drebrin family of proteins that are developmentally regulated in the brain. A decrease in the amount of this protein in the brain has been implicated as a possible contributing factor in the pathogenesis of memory disturbance in Alzheimer's disease. At least two alternative splice variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Function	Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites. Required for actin polymerization at immunological synapses (IS) and for CXCR4 recruitment to IS. [UniProt]
Cellular Localization	Cytoplasm, cell cortex. Cell junction. Cell projection, growth cone. Note=In the absence of antigen, evenly distributed throughout subcortical regions of the T-cell membrane and cytoplasm. In the presence of antigen, distributes to the immunological synapse forming at the T-cell-APC contact area, where it localizes at the peripheral and distal supramolecular activation clusters. Colocalized with DBN1, RUFY3 and F-actin at the transitional domain of the axonal growth cone. [UniProt]
Calculated MW	71 kDa

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