ARG40492

anti-Doublecortin antibody

anti-Doublecortin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Controls and Markers antibody; Neuroscience antibody; Hippocampal Neurogenesis Marker antibody; Immature Neuronal Cells Marker antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Doublecortin
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Doublecortin
Antigen Species Human
Immunogen Recombinant protein of human Doublecortin.
Conjugation Un-conjugated
Alternate Names LISX; Doublin; SCLH; Lissencephalin-X; Neuronal migration protein doublecortin; DC; Lis-X; DBCN; XLIS

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50
IHC-P1:20
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer 50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Concentration Batch dependent
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13193 Mouse DCX

GeneID: 1641 Human DCX

Swiss-port # O43602 Human Neuronal migration protein doublecortin

Swiss-port # O88809 Mouse Neuronal migration protein doublecortin

Gene Symbol DCX
Gene Full Name doublecortin
Background This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]
Function Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration. [UniProt]
Cellular Localization Cytoplasm. Cell projection. Note=Localizes at neurite tips. [UniProt]
Research Area Controls and Markers antibody; Neuroscience antibody; Hippocampal Neurogenesis Marker antibody; Immature Neuronal Cells Marker antibody
Calculated MW 41 kDa
PTM Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules (By similarity). Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21 (By similarity).

Ubiquitinated by MDM2, leading to its degradation by the proteasome. Ubiquitinated by MDM2 and subsequent degradation leads to reduce the dendritic spine density of olfactory bulb granule cells. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG40492 anti-Doublecortin antibody WB image

    Western blot: Rat brain lysate stained with ARG40492 anti-Doublecortin antibody at 1:1000 dilution.