ARG52265
anti-Dopamine Transporter phospho (Thr53) antibody
anti-Dopamine Transporter phospho (Thr53) antibody for Immunohistochemistry,Western blot and Rat
Neuroscience antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Dopamine Transporter phospho (Thr53) |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | IHC, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Dopamine Transporter |
| Antigen Species | Rat |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | PKDYS; Sodium-dependent dopamine transporter; Solute carrier family 6 member 3; DAT1; DAT; DA transporter |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~55k glycosylated form of the DAT protein phosphorylated at Thr53. Relative mobility may vary depending on the state of glycosylation of the DAT protein. The antibody works best in lysates that have not been boiled prior to being run on an SDS-PAGE gel. Immunolabeling of the DAT band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P23977 Rat Sodium-dependent dopamine transporter |
|---|---|
| Gene Symbol | SLC6A3 |
| Gene Full Name | solute carrier family 6 (neurotransmitter transporter), member 3 |
| Background | The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996). It has been shown that phosphorylation at Thr53 directly affects dopamine influx and amphetamine-stimulated substrate efflux, indicating that the Thr53 residue plays a major role in transport activity (Foster et al., 2012). |
| Highlight | Related Antibody Duos and Panels: ARG30102 Phospho Dopamine Transporter Antibody Duo (Total, pT53) Related products: Dopamine Transporter antibodies; Dopamine Transporter Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Neuroscience antibody |
| Calculated MW | 68 kDa |
Images (1) Click the Picture to Zoom In
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ARG52265 anti-Dopamine Transporter phospho (Thr53) antibody WB image
Western blot: Rat caudate lysate with (lane 2) or without (lane 1) phospho-peptide stained with ARG52265 anti-Dopamine Transporter phospho (Thr53) antibody.
