ARG65281
anti-Desmoplakin I antibody
anti-Desmoplakin I antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Desmoplakin isoform I |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | IHC-P |
| Specificity | This antibody is expected to recognize reported isoform I (NP_004406.2) only. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Desmoplakin I |
| Antigen Species | Human |
| Immunogen | C-QESVKQAHLRNEH |
| Conjugation | Un-conjugated |
| Alternate Names | 250/210 kDa paraneoplastic pemphigus antigen; Desmoplakin; DP; DPII; DCWHKTA; DPI |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is an obligate component of functional desmosomes that anchors intermediate filaments to desmosomal plaques. The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds with intermediate filaments. In the mid-region of desmoplakin, a coiled-coiled rod domain is responsible for homodimerization. Mutations in this gene are the cause of several cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus. [provided by RefSeq, Jul 2008] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 332 kDa |
| PTM | Ser-2849 is probably phosphorylated by a cAMP-dependent protein kinase. Phosphorylation on Ser-2849 probably affects its association with epidermal, simple cytokeratins and VIM intermediate filaments. |
Images (1) Click the Picture to Zoom In
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ARG65281 anti-Desmoplakin antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Heart. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG65281 anti-Desmoplakin (aa 1672 -1684) antibody at 3.8 µg/ml dilution followed by AP-staining.
