ARG52941

anti-Desmin antibody

anti-Desmin antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody; Smooth Muscle Marker antibody; Cardiomyocyte Cell Surface Marker antibody; Mural Cell Marker antibody; Microvascular Density Study antibody
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Overview

Product Description Rabbit Polyclonal antibody recognizes Desmin
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Desmin
Antigen Species Human
Immunogen Synthetic peptide mapping near the C-terminus of human desmin.
Conjugation Un-conjugated
Alternate Names LGMD2R; CSM1; Desmin; CSM2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:200
IPAssay-Dependent
WBAssay-dependent
Application Note IHC-P:
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Sarcomas, Skeletal Muscle

Properties

Form Liquid
Purification Immunogen affinity purified
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13346 Mouse DES

GeneID: 1674 Human DES

GeneID: 64362 Rat DES

Gene Full Name desmin
Background Desmin is a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq, Jul 2008]
Function Desmin: Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed:25358400). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed:24200904, PubMed:25394388, PubMed:26724190). May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin. [UniProt]
Cellular Localization Cytoplasm
Highlight Related Antibody Duos and Panels:
ARG30051 Smooth Muscle Marker Antibody Duo (Desmin, SMA)
ARG30142 Cardiomyocyte Cell Surface Marker Antibody Panel (Cx43, Desmin, SIRP)
Related products:
Desmin antibodies; Desmin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody; Smooth Muscle Marker antibody; Cardiomyocyte Cell Surface Marker antibody; Mural Cell Marker antibody; Microvascular Density Study antibody
Calculated MW 54 kDa
PTM ADP-ribosylation prevents ability to form intermediate filaments.

Images (2) Click the Picture to Zoom In

  • ARG52941 anti-Desmin antibody WB image

    Western blot: 30 µg of Rat heart lysate stained with ARG52941 anti-Desmin antibody at 1:500 dilution.

  • ARG52941 anti-Desmin antibody IHC-P image

    Immunohistochemistry: Human Skeletal Muscle stained with ARG52941 anti-Desmin antibody

Customer's Feedback

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Review for anti-Desmin antibody

Application:WB

Sample:Mouse heart

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC