ARG52941
anti-Desmin antibody
anti-Desmin antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody; Smooth Muscle Marker antibody; Cardiomyocyte Cell Surface Marker antibody; Mural Cell Marker antibody; Microvascular Density Study antibody
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Overview
| Product Description | Rabbit Polyclonal antibody recognizes Desmin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Desmin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide mapping near the C-terminus of human desmin. |
| Conjugation | Un-conjugated |
| Alternate Names | LGMD2R; CSM1; Desmin; CSM2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Incubation Time: 10 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Sarcomas, Skeletal Muscle |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunogen affinity purified |
| Buffer | PBS (pH 7.6), 1% BSA and <0.1% Sodium azide |
| Preservative | <0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Full Name | desmin |
| Background | Desmin is a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq, Jul 2008] |
| Function | Desmin: Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed:25358400). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed:24200904, PubMed:25394388, PubMed:26724190). May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin. [UniProt] |
| Cellular Localization | Cytoplasm |
| Highlight | Related Antibody Duos and Panels: ARG30051 Smooth Muscle Marker Antibody Duo (Desmin, SMA) ARG30142 Cardiomyocyte Cell Surface Marker Antibody Panel (Cx43, Desmin, SIRP) Related products: Desmin antibodies; Desmin Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Pericytes, new therapeutic target for Alzheimer's disease? |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody; Smooth Muscle Marker antibody; Cardiomyocyte Cell Surface Marker antibody; Mural Cell Marker antibody; Microvascular Density Study antibody |
| Calculated MW | 54 kDa |
| PTM | ADP-ribosylation prevents ability to form intermediate filaments. |
Images (2) Click the Picture to Zoom In
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ARG52941 anti-Desmin antibody WB image
Western blot: 30 µg of Rat heart lysate stained with ARG52941 anti-Desmin antibody at 1:500 dilution.
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ARG52941 anti-Desmin antibody IHC-P image
Immunohistochemistry: Human Skeletal Muscle stained with ARG52941 anti-Desmin antibody
Customer's Feedback
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Review for anti-Desmin antibody
Application：WB
Sample：Mouse heart
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
