ARG59576
anti-DUSP5 antibody
anti-DUSP5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DUSP5 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DUSP5 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-280 of Human DUSP5 (NP_004424.3). |
| Conjugation | Un-conjugated |
| Alternate Names | ERT; Epithelium-specific Ets transcription factor 1; Epithelial-restricted with serine box; ESE-1; ESX; E74-like factor 3; EPR-1; Epithelium-restricted Ets protein ESX; ETS-related transcription factor Elf-3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | A549 | ||||||
| Observed Size | 42 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ELF3 |
| Gene Full Name | E74-like factor 3 (ets domain transcription factor, epithelial-specific ) |
| Function | Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development (By similarity). [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. Note=Localizes to the cytoplasm where it has been shown to transform MCF-12A mammary epithelial cells via a novel cytoplasmic mechanism. Also transiently expressed and localized to the nucleus where it induces apoptosis in non-transformed breast epithelial cells MCF-10A and MCF-12A via a transcription-dependent mechanism. [UniProt] |
| Calculated MW | 41 kDa |
Images (2) Click the Picture to Zoom In
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ARG59576 anti-DUSP5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney stained with ARG59576 anti-DUSP5 antibody at 1:200 dilution.
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ARG59576 anti-DUSP5 antibody WB image
Western blot: 25 µg of A549 cell lysate stained with ARG59576 anti-DUSP5 antibody at 1:1000 dilution.
