ARG57052
anti-DUSP3 antibody [9E6]
anti-DUSP3 antibody [9E6] for Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [9E6] recognizes DUSP3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 9E6 |
| Isotype | IgG2b, kappa |
| Target Name | DUSP3 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-185 of Human DUSP3. |
| Conjugation | Un-conjugated |
| Alternate Names | EC 3.1.3.16; Vaccinia H1-related phosphatase; Dual specificity protein phosphatase 3; EC 3.1.3.48; VHR; Dual specificity protein phosphatase VHR |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P51452 Human Dual specificity protein phosphatase 3 |
|---|---|
| Gene Symbol | DUSP3 |
| Gene Full Name | dual specificity phosphatase 3 |
| Background | The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq, Jul 2008] |
| Function | Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2. [UniProt] |
| Calculated MW | 20 kDa |
Images (1) Click the Picture to Zoom In
-
ARG57052 anti-DUSP3 antibody [9E6] WB image
Western blot: 40 µg of 1) HeLa cell lysate, 2) HepG2 cell lysate, 3) Jurkat cell lysate, 4) MCF7 cell lysate, 5) 293T cell lysate, 6) U87MG cell lysate, 7) K562 cell lysate stained with ARG57052 anti-DUSP3 antibody [9E6] at 1:3000.
