ARG66274
anti-DPYSL2 / CRMP2 phospho (Ser522) antibody
anti-DPYSL2 / CRMP2 phospho (Ser522) antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DPYSL2 / CRMP2 phospho (Ser522) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | The antibody detects endogenous levels of CRMP2 only when phosphorylated at serine 522. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DPYSL2 / CRMP2 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated phospho-specific peptide around Ser522 (KTS(p)PA) of Human CRMP2. |
| Conjugation | Un-conjugated |
| Alternate Names | Unc-33-like phosphoprotein 2; Dihydropyrimidinase-related protein 2; Collapsin response mediator protein 2; DRP2; ULIP2; N2A3; DRP-2; DHPRP2; ULIP-2; CRMP-2; CRMP2 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with phospho-specific peptide and the non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. |
| Buffer | PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q16555 Human Dihydropyrimidinase-related protein 2 |
|---|---|
| Gene Symbol | DPYSL2 |
| Gene Full Name | dihydropyrimidinase-like 2 |
| Background | This gene encodes a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. The encoded protein promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimer's disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011] |
| Function | Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis. [UniProt] |
| Calculated MW | 62 kDa |
| PTM | 3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509. Phosphorylation at Thr-514 by GSK3B abolishes tubulin-binding leading to destabilization of microtubule assembly in axons and neurodegeneration (By similarity). Phosphorylation by DYRK2 at Ser-522 is required for subsequent phosphorylation by GSK3B. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG66274 anti-DPYSL2 / CRMP2 phospho (Ser522) antibody WB image
Western blot: HUVEC cells treated or untreated with antigen-specific peptide. The blots were stained with ARG66274 anti-DPYSL2 / CRMP2 phospho (Ser522) antibody.
