ARG64722
anti-DPM1 antibody
anti-DPM1 antibody for Western blot and Rat
Controls and Markers antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes DPM1 |
|---|---|
| Tested Reactivity | Rat |
| Predict Reactivity | Ms |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DPM1 |
| Antigen Species | Mouse |
| Immunogen | PQGRSSRQDKYS-C |
| Conjugation | Un-conjugated |
| Alternate Names | Mannose-P-dolichol synthase subunit 1; Dolichol-phosphate mannosyltransferase subunit 1; Dolichyl-phosphate beta-D-mannosyltransferase subunit 1; DPM synthase subunit 1; EC 2.4.1.83; MPD synthase subunit 1; MPDS; CDGIE; Dolichol-phosphate mannose synthase subunit 1 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | Dpm1 |
|---|---|
| Gene Full Name | dolichol-phosphate (beta-D) mannosyltransferase 1 |
| Background | Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. [provided by RefSeq, Jul 2008] |
| Function | Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins; catalytic subunit of the dolichol-phosphate mannose (DPM) synthase complex. [UniProt] |
| Research Area | Controls and Markers antibody |
| Calculated MW | 30 kDa |
Images (1) Click the Picture to Zoom In
-
ARG64722 anti-DPM1 antibody WB image
Western blot: Rat Liver lysate (35 µg protein in RIPA buffer) stained with ARG64722 anti-DPM1 antibody at 0.05 µg/ml dilution.
