ARG63676
anti-DOK5 antibody
anti-DOK5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes DOK5 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog, Pig |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DOK5 |
| Antigen Species | Human |
| Immunogen | CLHRTETFPAYRSEH |
| Conjugation | Un-conjugated |
| Alternate Names | IRS6; IRS-6; Docking protein 5; C20orf180; Insulin receptor substrate 6; Downstream of tyrosine kinase 5 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Microwaved tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. In contrast to other DOK family proteins, this protein does not interact with RASGAP. [provided by RefSeq, Jul 2008] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 35 kDa |
| PTM | Phosphorylated on tyrosine residues in response to insulin, IGF1 and GDNF. |
Images (2) Click the Picture to Zoom In
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ARG63676 anti-DOK5 antibody WB image
Western Blot: 1). Mock transfection; 2) DOK5 (RC211718) expressing plasmid transfected HEK293 cell lysate standed with ARG63676 anti-DOK5 antibody
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ARG63676 anti-DOK5 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Tonsil. (Microwaved antigen retrieval with citrate buffer pH6) stained with ARG63676 anti-DOK5 antibody at 1 µg/ml dilution followed by HRP-staining.
