ARG45195
anti-DNAJC10 antibody
anti-DNAJC10 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DNAJC10 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DNAJC10 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human DNAJC10. |
| Conjugation | Un-conjugated |
| Alternate Names | DNAJC10; DnaJ Heat Shock Protein Family (Hsp40) Member C10; ERdj5; PDIA19; Endoplasmic Reticulum DNA J Domain-Containing Protein 5; Protein Disulfide Isomerase Family A, Member 19; DnaJ (Hsp40) Homolog, Subfamily C, Member 10; DnaJ Homolog Subfamily C Member 10; ER-Resident Protein ERdj5; Macrothioredoxin; MTHr; J-Domain-Containing Protein Disulfide Isomerase-Like Protein 3; EC 1.8.4.-; ERDJ5; JPDI_x000D_ |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 91 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DNAJC10 |
| Gene Full Name | DnaJ Heat Shock Protein Family (Hsp40) Member C10 |
| Background | This gene encodes an endoplasmic reticulum co-chaperone which is part of the endoplasmic reticulum-associated degradation complex involved in recognizing and degrading misfolded proteins. The encoded protein reduces incorrect disulfide bonds in misfolded glycoproteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2012] |
| Function | Endoplasmic reticulum disulfide reductase involved both in the correct folding of proteins and degradation of misfolded proteins. Required for efficient folding of proteins in the endoplasmic reticulum by catalyzing the removal of non-native disulfide bonds formed during the folding of proteins, such as LDLR. Also involved in endoplasmic reticulum-associated degradation (ERAD) by reducing incorrect disulfide bonds in misfolded glycoproteins recognized by EDEM1. Interaction with HSPA5 is required its activity, not for the disulfide reductase activity, but to facilitate the release of DNAJC10 from its substrate. Promotes apoptotic signaling pathway in response to endoplasmic reticulum stress.. [UniProt] |
| Cellular Localization | Endoplasmic reticulum. [UniProt] |
| Calculated MW | 91 kDa |
| PTM | Disulfide bond; Glycoprotein. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG45195 anti-DNAJC10 antibody IHC-P image
Immunohistochemistry: Human gastric cancer stained with ARG45195 anti-DNAJC10 antibody at 2 μg/ml dilution.
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ARG45195 anti-DNAJC10 antibody ICC/IF image
Immunofluorescence: A431 stained with ARG45195 anti-DNAJC10 antibody at 5 ug/ml dilution.
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ARG45195 anti-DNAJC10 antibody WB image
Western blot: Hela, HEPG2, and U87 stained with ARG45195 anti-DNAJC10 antibody at 0.5 μg/ml dilution.
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ARG45195 anti-DNAJC10 antibody FACS image
Flow Cytometry: Hela stained with ARG45195 anti-DNAJC10 antibody at 1 µg/10^6 cells dilution.
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ARG45195 anti-DNAJC10 antibody WB image
Western blot: Rat stomach and RH35 stained with ARG45195 anti-DNAJC10 antibody at 0.5 μg/ml dilution.
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ARG45195 anti-DNAJC10 antibody WB image
Western blot: Mouse stomach and HEPA1-6 stained with ARG45195 anti-DNAJC10 antibody at 0.5 μg/ml dilution.
