ARG43061
anti-DNAJA2 antibody
anti-DNAJA2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DNAJA2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DNAJA2 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to M1-N140 of Human DNAJA2. |
| Conjugation | Un-conjugated |
| Alternate Names | Dnj3; DJ3; Dj3; Cell cycle progression restoration gene 3 protein; DnaJ homolog subfamily A member 2; PRO3015; RDJ2; DJA2; HIRA-interacting protein 4; DNJ3; HIRIP4; DNAJ; CPR3; Renal carcinoma antigen NY-REN-14 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DNAJA2 |
| Gene Full Name | DnaJ (Hsp40) homolog, subfamily A, member 2 |
| Background | The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro. [provided by RefSeq, Jul 2008] |
| Function | Co-chaperone of Hsc70. Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed:24318877). [UniProt] |
| Cellular Localization | Membrane; Lipid-anchor. [UniProt] |
| Calculated MW | 46 kDa |
Images (8) Click the Picture to Zoom In
-
ARG43061 anti-DNAJA2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human rectal cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43061 anti-DNAJA2 antibody at 1 µg/ml dilution, overnight at 4°C.
-
ARG43061 anti-DNAJA2 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Jurkat, HepG2, K562, Raji, Rat kidney, Mouse testis and NIH/3T3 whole cell lysates stained with ARG43061 anti-DNAJA2 antibody at 0.5 µg/ml dilution, overnight at 4°C.
-
ARG43061 anti-DNAJA2 antibody FACS image
Flow Cytometry: A431 cells were blocked with 10% normal goat serum and then stained with ARG43061 anti-DNAJA2 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
-
ARG43061 anti-DNAJA2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse testis tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43061 anti-DNAJA2 antibody at 1 µg/ml dilution, overnight at 4°C.
-
ARG43061 anti-DNAJA2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat testis tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43061 anti-DNAJA2 antibody at 1 µg/ml dilution, overnight at 4°C.
-
ARG43061 anti-DNAJA2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testicular cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43061 anti-DNAJA2 antibody at 1 µg/ml dilution, overnight at 4°C.
-
ARG43061 anti-DNAJA2 antibody FACS image
Flow Cytometry: ANA-1 cells were blocked with 10% normal goat serum and then stained with ARG43061 anti-DNAJA2 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
-
ARG43061 anti-DNAJA2 antibody FACS image
Flow Cytometry: NRK cells were blocked with 10% normal goat serum and then stained with ARG43061 anti-DNAJA2 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
