ARG59503
anti-DNA polymerase beta antibody
anti-DNA polymerase beta antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DNA polymerase beta |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DNA polymerase beta |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-335 of Human DNA polymerase beta (NP_002681.1). |
| Conjugation | Un-conjugated |
| Alternate Names | EC 4.2.99.-; EC 2.7.7.7; DNA polymerase beta |
Application Instructions
| Predict Reactivity Note | Rat | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Suggestion |
|
||||||||
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Jurkat | ||||||||
| Observed Size | 37 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | POLB |
| Gene Full Name | polymerase (DNA directed), beta |
| Background | The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011] |
| Function | Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm. Note=Cytoplasmic in normal conditions. Translocates to the nucleus following DNA damage. [UniProt] |
| Calculated MW | 38 kDa |
| PTM | Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity. Ubiquitinated at Lys-41, Lys-61 and Lys-81: monoubiquitinated by HUWE1/ARF-BP1. Monoubiquitinated protein is then the target of STUB1/CHIP, which catalyzes polyubiquitination from monoubiquitin, leading to degradation by the proteasome. USP47 mediates the deubiquitination of monoubiquitinated protein, preventing polyubiquitination by STUB1/CHIP and its subsequent degradation. [UniProt] |
Images (3) Click the Picture to Zoom In
-
ARG59503 anti-DNA polymerase beta antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG59503 anti-DNA polymerase beta antibody.
-
ARG59503 anti-DNA polymerase beta antibody WB image
Western blot: 25 µg of Jurkat cell lysate stained with ARG59503 anti-DNA polymerase beta antibody at 1:1000 dilution.
-
ARG59503 anti-DNA polymerase beta antibody WB image
Western blot: 25 µg of extracts from normal (control) and POLB knockout (KO) HeLa cells, using ARG59503 anti-DNA polymerase beta antibody at 1:1000 dilution.
