ARG54096
anti-DLAT antibody
anti-DLAT antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse
Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes DLAT |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | DLAT |
| Antigen Species | Human |
| Immunogen | Purified recombinant human DLAT protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; PDC-E2; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; M2 antigen complex 70 kDa subunit; EC 2.3.1.12; PBC; Pyruvate dehydrogenase complex component E2; DLTA; 70 kDa mitochondrial autoantigen of primary biliary cirrhosis; PDCE2 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol |
| Preservative | 0.2% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P10515 Human Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydr Swiss-port # Q8BMF4 Mouse Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydr |
|---|---|
| Gene Symbol | DLAT |
| Gene Full Name | dihydrolipoamide S-acetyltransferase |
| Background | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). |
| Function | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. [UniProt] |
| Cellular Localization | Mitochondrion matrix. |
| Research Area | Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 69 kDa |
| PTM | Delipoylated at Lys-132 and Lys-259 by SIRT4, delipoylation decreases the PHD complex activity. |
Images (3) Click the Picture to Zoom In
-
ARG54096 anti-DLAT antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG54096 anti-DLAT antibody at 1:300 dilution.
-
ARG54096 anti-DLAT antibody WB image
Western blot: Jurkat cell lysate stained with ARG54096 anti-DLAT antibody at 1:1000 dilution.
-
ARG54096 anti-DLAT antibody IP image
Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG54096 anti-DLAT antibody.
