ARG63313
anti-DKK3 / REIC antibody
anti-DKK3 / REIC antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Developmental Biology antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes DKK3 / REIC |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Specificity | NCBI has removed RIG (GeneID 10530) and has it replaced by DKK3 (GeneID 27122). The AAC35996 protein is no longer represented by an NP RefSeq code. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DKK3 / REIC |
| Antigen Species | Human |
| Immunogen | TLSVVGPHPKPCIL |
| Conjugation | Un-conjugated |
| Alternate Names | Dickkopf-3; REIC; Dkk-3; RIG; hDkk-3; Dickkopf-related protein 3 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DKK3 |
| Gene Full Name | dickkopf WNT signaling pathway inhibitor 3 |
| Background | This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008] |
| Function | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity). [UniProt] |
| Research Area | Developmental Biology antibody; Signaling Transduction antibody |
| Calculated MW | 38 kDa |
| PTM | N- and O-glycosylated. |
Images (1) Click the Picture to Zoom In
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ARG63313 anti-DKK3 / REIC antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Uterus. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63313 anti-DKK3 / REIC antibody at 3.8 µg/ml dilution followed by AP-staining.
