ARG63198
anti-DKK3 / REIC antibody
anti-DKK3 / REIC antibody for Western blot and Human
Developmental Biology antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes DKK3 / REIC |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | NP_037385.2; NP_056965.3 and NP_001018067.1 are varients of the same protein. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DKK3 / REIC |
| Antigen Species | Human |
| Immunogen | C-AAAALLGGEEI |
| Conjugation | Un-conjugated |
| Alternate Names | Dickkopf-3; REIC; Dkk-3; RIG; hDkk-3; Dickkopf-related protein 3 |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008] |
| Research Area | Developmental Biology antibody; Signaling Transduction antibody |
| Calculated MW | 38 kDa |
| PTM | N- and O-glycosylated. |
Images (2) Click the Picture to Zoom In
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ARG63198 anti-DKK3 / REIC antibody WB image
Western Blot: HEK293 lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63198 anti-DKK3 / REIC antibody at 2 µg/ml dilution.
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ARG63198 anti-DKK3 / REIC antibody WB image
Western blot: 35 µg of Human cerebellum lysate (in RIPA buffer) stained with ARG63198 anti-DKK3 / REIC antibody at 2 µg/ml dilution and incubated at RT for 1 hour.
