ARG64196
anti-DKC1 antibody
anti-DKC1 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes DKC1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms |
| Tested Application | IHC-P |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DKC1 |
| Antigen Species | Human |
| Immunogen | C-KRKRESESESDETPP |
| Conjugation | Un-conjugated |
| Alternate Names | NAP57; Dyskerin; CBF5; Nucleolar protein family A member 4; NOLA4; CBF5 homolog; H/ACA ribonucleoprotein complex subunit 4; Nucleolar protein NAP57; DKC; XAP101; Nopp140-associated protein of 57 kDa; snoRNP protein DKC1; DKCX; EC 5.4.99.- |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O60832 Human H/ACA ribonucleoprotein complex subunit 4 |
|---|---|
| Background | This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the NOLA1, 2 and 3 proteins. The protein encoded by this gene and the three NOLA proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The protein encoded by this gene is related to the Saccharomyces cerevisiae Cbf5p and Drosophila melanogaster Nop60B proteins. The gene lies in a tail-to-tail orientation with the palmitoylated erythrocyte membrane protein gene and is transcribed in a telomere to centromere direction. Both nucleotide substitutions and single trinucleotide repeat polymorphisms have been found in this gene. Mutations in this gene cause X-linked dyskeratosis congenita, a disease resulting in reticulate skin pigmentation, mucosal leukoplakia, nail dystrophy, and progressive bone marrow failure in most cases. Mutations in this gene also cause Hoyeraal-Hreidarsson syndrome, which is a more severe form of dyskeratosis congenita. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 58 kDa |
Images (1) Click the Picture to Zoom In
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ARG64196 anti-DKC1 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Spleen. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64196 anti-DKC1 antibody at 5 µg/ml dilution followed by AP-staining.
