ARG45523
anti-DKC1 antibody
anti-DKC1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DKC1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DKC1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human DKC1. |
| Conjugation | Un-conjugated |
| Alternate Names | DKC1; Dyskerin Pseudouridine Synthase 1; NOLA4; Dyskerin; XAP101; NAP57; Cbf5; H/ACA Ribonucleoprotein Complex Subunit DKC1; H/ACA Ribonucleoprotein Complex Subunit 4; Nopp140-Associated Protein Of 57 KDa; Nucleolar Protein Family A Member 4; Dyskeratosis Congenita 1, Dyskerin; Nucleolar Protein NAP57; SnoRNP Protein DKC1; CBF5 Homolog; DKC; EC 5.4.99.-; EC 5.4.99; CHINE1; DKCX |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 58 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DKC1 |
| Gene Full Name | Dyskerin Pseudouridine Synthase 1 |
| Background | This gene functions in two distinct complexes. It plays an active role in telomerase stabilization and maintenance, as well as recognition of snoRNAs containing H/ACA sequences which provides stability during biogenesis and assembly into H/ACA small nucleolar RNA ribonucleoproteins (snoRNPs). This gene is highly conserved and widely expressed, and may play additional roles in nucleo-cytoplasmic shuttling, DNA damage response, and cell adhesion. Mutations have been associated with X-linked dyskeratosis congenita. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| Function | [Isoform 1]: Catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. [UniProt] |
| Cellular Localization | Cytoplasm; Nucleus. [UniProt] |
| Calculated MW | 58 kDa |
| PTM | Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG45523 anti-DKC1 antibody IHC-P image
Immunohistochemistry: Human breast cancer stained with ARG45523 anti-DKC1 antibody at 2 μg/ml dilution.
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ARG45523 anti-DKC1 antibody ICC/IF image
Immunofluorescence: U20S stained with ARG45523 anti-DKC1 antibody at 5 μg/ml dilution.
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ARG45523 anti-DKC1 antibody WB image
Western blot: HEL stained with ARG45523 anti-DKC1 antibody at 0.5 μg/ml dilution.
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ARG45523 anti-DKC1 antibody FACS image
Flow Cytometry: A431 stained with ARG45523 anti-DKC1 antibody at 1 µg/10^6 cells dilution.
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ARG45523 anti-DKC1 antibody WB image
Western blot: Rat testis stained with ARG45523 anti-DKC1 antibody at 0.5 μg/ml dilution.
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ARG45523 anti-DKC1 antibody WB image
Western blot: Mouse testis stained with ARG45523 anti-DKC1 antibody at 0.5 μg/ml dilution.
