ARG64920

anti-DHX9 antibody

anti-DHX9 antibody for Western blot and Human

Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes DHX9
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name DHX9
Antigen Species Human
Immunogen TEGRNALIHKSSVNC
Conjugation Un-conjugated
Alternate Names NDHII; Nuclear DNA helicase II; Leukophysin; ATP-dependent RNA helicase A; NDH2; NDH II; EC 3.6.4.13; RHA; DEAH box protein 9; DDX9; LKP

Application Instructions

Application Suggestion
Tested Application Dilution
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1660 Human DHX9

Swiss-port # Q08211 Human ATP-dependent RNA helicase A

Background This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 11 and 13.[provided by RefSeq, Feb 2010]
Research Area Gene Regulation antibody
Calculated MW 141 kDa
PTM Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
May be phosphorylated by PRKDC/XRCC7. Phosphorylated by EIF2AK2/PKR and this phosphorylation perturbs its association with dsRNA.

Images (1) Click the Picture to Zoom In

  • ARG64920 anti-DHX9 antibody WB image

    Western blot: 35 µg of nuclear HeLa lysate stained with ARG64920 anti-DHX9 antibody at 1 µg/ml dilution.