ARG45441
anti-DGCR8 antibody
anti-DGCR8 antibody for IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DGCR8 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DGCR8 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human DGCR8. |
| Conjugation | Un-conjugated |
| Alternate Names | DGCR8; DGCR8 Microprocessor Complex Subunit; DGCRK6; C22orf12; Pasha; Gy1; DiGeorge Syndrome Critical Region Gene 8; Microprocessor Complex Subunit DGCR8; DiGeorge Syndrome Critical Region 8; DGCR8, Microprocessor Complex Subunit; Chromosome 22 Open Reading Frame 12 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 100 kDa |
Properties
| Form | Powder |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8WYQ5 Human Microprocessor complex subunit DGCR8 Swiss-port # Q9EQM6 Mouse Microprocessor complex subunit DGCR8 |
|---|---|
| Gene Symbol | DGCR8 |
| Gene Full Name | DGCR8 Microprocessor Complex Subunit |
| Background | This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010] |
| Function | Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 86 kDa |
| PTM | Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt] |
