ARG64152
anti-DGAT2 antibody
anti-DGAT2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes DGAT2 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Dog |
| Tested Application | FACS, IHC-P |
| Specificity | This antibody is not expected to cross-react with other DGAT members. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DGAT2 |
| Antigen Species | Human |
| Immunogen | C-KLEHPTQQDIDLYH |
| Conjugation | Un-conjugated |
| Alternate Names | Diacylglycerol O-acyltransferase 2; HMFN1045; ARAT; Retinol O-fatty-acyltransferase; EC 2.3.1.76; Diglyceride acyltransferase 2; Acyl-CoA retinol O-fatty-acyltransferase; EC 2.3.1.20; GS1999FULL |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q96PD7 Human Diacylglycerol O-acyltransferase 2 |
|---|---|
| Background | This gene encodes one of two enzymes which catalyzes the final reaction in the synthesis of triglycerides in which diacylglycerol is covalently bound to long chain fatty acyl-CoAs. The encoded protein catalyzes this reaction at low concentrations of magnesium chloride while the other enzyme has high activity at high concentrations of magnesium chloride. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
| Research Area | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 44 kDa |
Images (2) Click the Picture to Zoom In
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ARG64152 anti-DGAT2 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Liver. (Microwaved antigen retrieval with Tris/EDTA buffer pH9) stained with ARG64152 anti-DGAT2 antibody at 3 µg/ml dilution followed by HRP-staining.
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ARG64152 anti-DGAT2 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG64152 anti-DGAT2 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
