ARG57517

anti-DDDDK tag antibody

anti-DDDDK tag antibody for Immunoprecipitation,Western blot and Other

Controls and Markers antibody; Tag Internal Control antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes DDDDK tag (Equivalent to Sigma's anti-FLAG antibodies).
Tested Reactivity Other
Tested Application IP, WB
Specificity DDDDK-tag antibody will detect amino- or carboxy-terminal DDDDK-tagged recombinant proteins.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name DDDDK tag
Antigen Species Others
Immunogen Synthetic peptide containing DYKDDDDK.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
IPAssay-dependent
WB0.5 - 1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to 1-2 weeks. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Research Area Controls and Markers antibody; Tag Internal Control antibody

Images (2) Click the Picture to Zoom In

  • ARG57517 anti-DDDDK tag antibody WB image

    Western blot: DDDDK tag-containing recombinant protein stained with ARG57517 anti-DDDDK tag antibody at (A) 0.125 and (B) 0.25 µg/ml dilution.

  • ARG57517 anti-DDDDK tag antibody IP image

    Immunoprecipitation: 20 ng of DDDDK-tagged recombinant GGP1 protein immunoprecipitated with ARG57517 anti-DDDDK tag antibody and then stained with 1 µg/ml of anti-GGP1 antibody.