ARG55726

anti-DDDDK tag antibody

anti-DDDDK tag antibody for Western blot and Other

Controls and Markers antibody; Tag Internal Control antibody

Overview

Product Description Goat Polyclonal antibody recognizes DDDDK tag (Equivalent to Sigma's anti-FLAG antibodies).
Tested Reactivity Other
Tested Application WB
Specificity This antibody recognizes recombinant proteins containing DDDDK epitope tag fused to either amino- or carboxy-terminal of targeted proteins in transfected cells.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name DDDDK tag
Antigen Species Others
Immunogen Purified recombinant peptide DYKDDDDK produced in E. coli.
Conjugation Un-conjugated
Alternate Names D-tag; ECS Epitope Tag; ECS-tag; FLAG-tag

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500-1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.05% Sodium azide and 20% Glycerol
Preservative 0.05% Sodium azide
Stabilizer 20% Glycerol
Concentration 3 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Research Area Controls and Markers antibody; Tag Internal Control antibody

Images (1) Click the Picture to Zoom In

  • ARG55726 anti-DDDDK tag antibody WB image

    Western blot: 100 µg of 293 cells transfected with Flag-poly Ub stained with ARG55726 anti-DDDDK tag antibody at 1:1000 dilution.