ARG55182
anti-DCLK1 antibody
anti-DCLK1 antibody for ELISA,Immunohistochemistry,Western blot and Human,Mouse,Rat
Neuroscience antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DCLK1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, IHC, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DCLK1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide (14 aa) within the last 50 aa of Human DCLK1. |
| Conjugation | Un-conjugated |
| Alternate Names | DCAMKL1; DCLK; Serine/threonine-protein kinase DCLK1; Doublecortin-like and CAM kinase-like 1; DCDC3A; EC 2.7.11.1; Doublecortin domain-containing protein 3A; CL1; Doublecortin-like kinase 1; CLICK1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Human Brain Tissue Lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O15075 Human Serine/threonine-protein kinase DCLK1 Swiss-port # Q9JLM8 Mouse Serine/threonine-protein kinase DCLK1 |
|---|---|
| Gene Symbol | DCLK1 |
| Gene Full Name | doublecortin-like kinase 1 |
| Background | DCLK1 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK1 mRNA, like that of the homologous DCLK2 and DCLK3, is highly expressed in adult brain, but only DCLK1 and DCLK2 transcripts are present in human fetal brain and the developing mouse embryo, suggesting that DCLK1 and DCLK2 may play roles in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a gene that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization. Unlike DCLK1, DCLK2 expression does not change in DCX-null mice. |
| Function | Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system. [UniProt] |
| Research Area | Neuroscience antibody |
| Calculated MW | 82 kDa |
Images (2) Click the Picture to Zoom In
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ARG55182 anti-DCLK1 antibody WB image
Western blot: human brain tissue lysate stained with ARG55182 anti-DCLK1 antibody at (A) 0.5 and (B) 1 ug/ml dilution.
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ARG55182 anti-DCLK1 antibody IHC image
Immunohistochemistry: DLCK1 in rat brain tissue stained with ARG55182 anti-DCLK1 antibody at 2.5 ug/ml dilution.
