ARG54843
anti-DCLK1 antibody
anti-DCLK1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Neuroscience antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DCLK1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DCLK1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 690-720 (C-terminus) of Human DCLK1. |
| Conjugation | Un-conjugated |
| Alternate Names | DCAMKL1; DCLK; Serine/threonine-protein kinase DCLK1; Doublecortin-like and CAM kinase-like 1; DCDC3A; EC 2.7.11.1; Doublecortin domain-containing protein 3A; CL1; Doublecortin-like kinase 1; CLICK1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Mouse heart |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O15075 Human Serine/threonine-protein kinase DCLK1 Swiss-port # Q9JLM8 Mouse Serine/threonine-protein kinase DCLK1 |
|---|---|
| Gene Symbol | DCLK1 |
| Gene Full Name | doublecortin-like kinase 1 |
| Background | This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.[provided by RefSeq, Sep 2010] |
| Function | Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system. [UniProt] |
| Research Area | Neuroscience antibody |
| Calculated MW | 82 kDa |
Images (2) Click the Picture to Zoom In
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ARG54843 anti-DCLK1 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human hepatocarcinoma stained with ARG54843 anti-DCLK1 antibody.
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ARG54843 anti-DCLK1 antibody WB image
Western blot: 35 µg of Mouse heart lysate stained with ARG54843 anti-DCLK1 antibody.
