ARG45612
anti-DBT antibody
anti-DBT antibody for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DBT |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DBT |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human DBT. |
| Conjugation | Un-conjugated |
| Alternate Names | DBT; BCATE2; Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial; EC 2.3.1.168; 52 kDa mitochondrial autoantigen of primary biliary cirrhosis; Branched chain 2-oxo-acid dehydrogenase complex component E2; BCOADC-E2; Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue; 2-methylpropanoyltransferase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P11182 Human Lipoamide acyltransferase component of branched-chain alpha-keto acid dehy Swiss-port # P53395 Mouse Lipoamide acyltransferase component of branched-chain alpha-keto acid dehy |
|---|---|
| Gene Symbol | DBT |
| Gene Full Name | Dihydrolipoamide Branched Chain Transacylase E2 |
| Background | The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008] |
| Function | The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component. [UniProt] |
| Cellular Localization | Mitochondrion. [UniProt] |
| Calculated MW | 53 kDa |
| PTM | Acetylation; Phosphoprotein. [UniProt] |
Images (5) Click the Picture to Zoom In
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ARG45612 anti-DBT antibody ICC/IF image
Immunofluorescence: U20S stained with ARG45612 anti-DBT antibody at 5 μg/ml dilution.
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ARG45612 anti-DBT antibody WB image
Western blot: SiHa and A431 stained with ARG45612 anti-DBT antibody at 0.5 μg/ml dilution.
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ARG45612 anti-DBT antibody FACS image
Flow Cytometry: SiHa stained with ARG45612 anti-DBT antibody at 1 µg/10^6 cells dilution.
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ARG45612 anti-DBT antibody WB image
Western blot: Rat stomach stained with ARG45612 anti-DBT antibody at 0.5 μg/ml dilution.
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ARG45612 anti-DBT antibody WB image
Western blot: Mouse stomach stained with ARG45612 anti-DBT antibody at 0.5 μg/ml dilution.
