ARG58491
anti-DBI antibody
anti-DBI antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DBI |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DBI |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-114 of Human DBI (NP_001171513.1). |
| Conjugation | Un-conjugated |
| Alternate Names | DBI; ACBP; Acyl-CoA-binding protein; Endozepine; ACBD1; Diazepam-binding inhibitor; CCK-RP; EP |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | NCI-H460 | ||||||||||
| Observed Size | 11 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DBI |
| Gene Full Name | diazepam binding inhibitor (GABA receptor modulator, acyl-CoA binding protein) |
| Background | This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| Function | Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor. [UniProt] |
| Cellular Localization | Endoplasmic reticulum, Golgi apparatus. [UniProt] |
| Calculated MW | 10 kDa |
Images (3) Click the Picture to Zoom In
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ARG58491 anti-DBI antibody ICC/IF image
Immunofluorescence: MCF-7 cells stained with ARG58491 anti-DBI antibody.
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ARG58491 anti-DBI antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate stained with ARG58491 anti-DBI antibody at 1:100 dilution.
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ARG58491 anti-DBI antibody WB image
Western blot: 25 µg of NCI-H460 cell lysate stained with ARG58491 anti-DBI antibody at 1:1000 dilution.
