ARG52257
anti-DARPP32 phospho (Ser137) antibody
anti-DARPP32 phospho (Ser137) antibody for Western blot and Rat
Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DARPP32 phospho (Ser137) |
|---|---|
| Tested Reactivity | Rat |
| Predict Reactivity | Hu, Ms, Bov, Dog, NHuPrm |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DARPP32 |
| Antigen Species | Rat |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser137 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | Dopamine- and cAMP-regulated neuronal phosphoprotein; DARPP32; DARPP-32; Protein phosphatase 1 regulatory subunit 1B |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | Specific for the ~32k DARPP-32 protein phosphorylated at Ser137. Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen but not by the dephospho-peptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q6J4I0 Rat Protein phosphatase 1 regulatory subunit 1B |
|---|---|
| Gene Symbol | PPP1R1B |
| Gene Full Name | protein phosphatase 1, regulatory (inhibitor) subunit 1B |
| Background | DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002). |
| Research Area | Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 23 kDa |
| PTM | Dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Phosphorylation of Thr-34 is required for activity. |
Images (1) Click the Picture to Zoom In
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ARG52257 anti-DARPP32 phospho (Ser137) antibody WB image
Western blot: Rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated Thr 137 (control) stained with ARG52257 anti-DARPP32 phospho (Ser137) antibody. In the second lane immunolabeling is blocked by preadsorption of the phospho-peptide used as antigen.
