ARG66973
anti-Cytokeratin (pan) antibody
anti-Cytokeratin (pan) antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Cytokeratin (pan) |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Specificity | Detects endogenous levels of total cytokeratin 5, 6, 7, 8, 14, 17, 18, 19 and 20. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Cytokeratin (pan) |
| Antigen Species | Human |
| Conjugation | Un-conjugated |
| Alternate Names | 67 kDa cytokeratin; KRT1A; EPPK; Keratin, type II cytoskeletal 1; EHK1; K1; Keratin-1; CK1; Cytokeratin-1; Hair alpha protein; Type-II keratin Kb1; CK-1; NEPPK; EHK |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | Skin, MCF7, A549, Colon | ||||||||||
| Observed Size | 50 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 0.025% ProClin 300 and 40% Glycerol. |
| Preservative | 0.025% ProClin 300 |
| Stabilizer | 20% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT1 |
| Gene Full Name | keratin 1, type II |
| Background | The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008] |
| Function | May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK. [UniProt] |
| Cellular Localization | Cell membrane. Note=Located on plasma membrane of neuroblastoma NMB7 cells. [UniProt] |
| Calculated MW | 66 kDa |
| PTM | Undergoes deimination of some arginine residues (citrullination). [UniProt] |
Images (7) Click the Picture to Zoom In
-
ARG66973 anti-Cytokeratin (pan) antibody ICC/IF image
Immunofluorescence: MCF7 cells were fixed with 4% paraformaldehyde at RT for 10 min, permeabilized with 0.1% NP-40 at RT for 10 min and then cells were blocked with 5% BSA at RT for 30 min and stained with ARG66973 anti-Cytokeratin (pan) antibody (green) at 1:400 dilution at 4°C for overnight. DAPI (blue) was used for nuclear staining.
-
ARG66973 anti-Cytokeratin (pan) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human gastric carcinoma tissue stained with ARG66973 anti-Cytokeratin (pan) antibody at 1:400 dilution.
-
ARG66973 anti-Cytokeratin (pan) antibody WB image
Western blot: A549, Jurkat cell lysate stained with ARG66973 anti-Cytokeratin (pan) antibody at 1:3000 dilution, overnight at 4°C.
-
ARG66973 anti-Cytokeratin (pan) antibody FACS image
Flow Cytometry: MCF7 cells stained with ARG66973 anti-Cytokeratin (pan) antibody at 1:350 dilution.
-
ARG66973 anti-Cytokeratin (pan) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse colon tissue stained with ARG66973 anti-Cytokeratin (pan) antibody at 1:400 dilution.
-
ARG66973 anti-Cytokeratin (pan) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse spleen tissue stained with ARG66973 anti-Cytokeratin (pan) antibody at 1:400 dilution.
-
ARG66973 anti-Cytokeratin (pan) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse lung tissue stained with ARG66973 anti-Cytokeratin (pan) antibody at 1:400 dilution.
