ARG56129
anti-Cytokeratin (pan) antibody [AE1 + AE3]
anti-Cytokeratin (pan) antibody [AE1 + AE3] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Signaling Transduction antibody; Carcinoma Marker antibody
Overview
| Product Description | Mouse Monoclonal antibody [AE1 + AE3] recognizes Cytokeratin (pan) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Specificity | Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This pan keratin antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3-6, CK8, CK10, CK14-16, and CK19. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | AE1 + AE3 |
| Isotype | IgG1 |
| Target Name | Cytokeratin (pan) |
| Antigen Species | Human |
| Immunogen | Human epidermal keratin |
| Conjugation | Un-conjugated |
| Alternate Names | 67 kDa cytokeratin; KRT1A; EPPK; Keratin, type II cytoskeletal 1; EHK1; K1; Keratin-1; CK1; Cytokeratin-1; Hair alpha protein; Type-II keratin Kb1; CK-1; NEPPK; EHK |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT1 |
| Gene Full Name | keratin 1, type II |
| Background | The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008] |
| Function | May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK. [UniProt] |
| Research Area | Signaling Transduction antibody; Carcinoma Marker antibody |
| Calculated MW | 67 kDa (CK1); 64 kDa (CK3); 59 kDa (CK4); 58 kDa (CK5); 56 kDa (CK6); 52 kDa (CK8); 56.5 kDa (CK10); 50 kDa (CK14); 50 kDa (CK15); 48 kDa (CK16) and 40 kDa (CK19). |
| PTM | Undergoes deimination of some arginine residues (citrullination). |
Images (3) Click the Picture to Zoom In
-
ARG56129 anti-Cytokeratin (pan) antibody [AE1 + AE3] FACS image
Flow Cytometry: MCF-7 cells stained with PE-conjugated ARG56129 anti-Cytokeratin (pan) antibody [AE1 + AE3] (red, same as ARG56130); Cells alone (black); Isotype control (green).
-
ARG56129 anti-Cytokeratin (pan) antibody [AE1 + AE3] IHC-P image
Immunohistochemistry: Colon carcinoma stained with ARG56129 anti-Cytokeratin (pan) antibody [AE1 + AE3].
-
ARG56129 anti-Cytokeratin (pan) antibody [AE1 + AE3] IHC-P image
Immunohistochemistry: Colon carcinoma stained with ARG56129 anti-Cytokeratin (pan) antibody [AE1 + AE3].
