ARG55962

anti-Cytokeratin 8 antibody [TS1]

anti-Cytokeratin 8 antibody [TS1] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [TS1] recognizes Cytokeratin 8
Tested Reactivity Hu
Species Does Not React With Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone TS1
Isotype IgG1
Target Name Cytokeratin 8
Antigen Species Human
Immunogen Keratin preparation from a Human carcinoma.
Epitope Within aa. 343-357
Conjugation Un-conjugated
Alternate Names Keratin, type II cytoskeletal 8; KO; CYK8; CK-8; Type-II keratin Kb8; K2C8; CARD2; Keratin-8; K8; CK8; Cytokeratin-8

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 2 µg/10^6 cells
ICC/IF2 - 5 µg/ml
IHC-P2 - 5 µg/ml
WB0.5 - 2 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control SKOV3
Observed Size ~ 45 kDa

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3856 Human KRT8

Swiss-port # P05787 Human Keratin, type II cytoskeletal 8

Gene Symbol KRT8
Gene Full Name keratin 8, type II
Background This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
Function Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt]
Cellular Localization Cytoplasmic
Calculated MW 54 kDa
PTM Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

Images (4) Click the Picture to Zoom In

  • ARG55962 anti-Cytokeratin 8 antibody [TS1] ICC/IF image

    Immunofluorescence: Permeabilized HeLa cells stained with ARG55962 anti-Cytokeratin 8 antibody [TS1] (green). Reddot (red) for nuclear staining.

  • ARG55962 anti-Cytokeratin 8 antibody [TS1] IHC-P image

    Immunohistochemistry: Human colon carcinoma stained with ARG55962 anti-Cytokeratin 8 antibody [TS1].

  • ARG55962 anti-Cytokeratin 8 antibody [TS1] WB image

    Western blot: SKOV3 cell lysate stained with ARG55962 anti-Cytokeratin 8 antibody [TS1].

  • ARG55962 anti-Cytokeratin 8 antibody [TS1] FACS image

    Flow Cytometry: Permeabilized HeLa cells stained with ARG55962 anti-Cytokeratin 8 antibody [TS1] (blue); Isotype control (red).