ARG55962
anti-Cytokeratin 8 antibody [TS1]
anti-Cytokeratin 8 antibody [TS1] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [TS1] recognizes Cytokeratin 8 |
|---|---|
| Tested Reactivity | Hu |
| Species Does Not React With | Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | TS1 |
| Isotype | IgG1 |
| Target Name | Cytokeratin 8 |
| Antigen Species | Human |
| Immunogen | Keratin preparation from a Human carcinoma. |
| Epitope | Within aa. 343-357 |
| Conjugation | Un-conjugated |
| Alternate Names | Keratin, type II cytoskeletal 8; KO; CYK8; CK-8; Type-II keratin Kb8; K2C8; CARD2; Keratin-8; K8; CK8; Cytokeratin-8 |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||||
| Positive Control | SKOV3 | ||||||||||
| Observed Size | ~ 45 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT8 |
| Gene Full Name | keratin 8, type II |
| Background | This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] |
| Function | Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt] |
| Cellular Localization | Cytoplasmic |
| Calculated MW | 54 kDa |
| PTM | Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. |
Images (4) Click the Picture to Zoom In
-
ARG55962 anti-Cytokeratin 8 antibody [TS1] ICC/IF image
Immunofluorescence: Permeabilized HeLa cells stained with ARG55962 anti-Cytokeratin 8 antibody [TS1] (green). Reddot (red) for nuclear staining.
-
ARG55962 anti-Cytokeratin 8 antibody [TS1] IHC-P image
Immunohistochemistry: Human colon carcinoma stained with ARG55962 anti-Cytokeratin 8 antibody [TS1].
-
ARG55962 anti-Cytokeratin 8 antibody [TS1] WB image
Western blot: SKOV3 cell lysate stained with ARG55962 anti-Cytokeratin 8 antibody [TS1].
-
ARG55962 anti-Cytokeratin 8 antibody [TS1] FACS image
Flow Cytometry: Permeabilized HeLa cells stained with ARG55962 anti-Cytokeratin 8 antibody [TS1] (blue); Isotype control (red).
