ARG62463
anti-Cytokeratin 7 antibody [OV-TL 12/30]
anti-Cytokeratin 7 antibody [OV-TL 12/30] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
CK7/CK20 Carcinoma Study antibody
Overview
| Product Description | Mouse Monoclonal antibody [OV-TL 12/30] recognizes Cytokeratin 7 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| Specificity | This antibody reacts with the 54 kD cytokeratin 7 intermediate filament protein |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | OV-TL 12/30 |
| Isotype | IgG1, kappa |
| Target Name | Cytokeratin 7 |
| Antigen Species | Human |
| Immunogen | OTN 11, ovarian carcinoma cell line |
| Conjugation | Un-conjugated |
| Alternate Names | SCL; Sarcolectin; CK-7; Cytokeratin-7; CK7; Keratin, type II cytoskeletal 7; Keratin-7; K7; K2C7; Type-II keratin Kb7 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | 20 mM tris-borate (pH 7.5), 150 mM sodium chloride, dialyzed media RPMI 1640/D-MEM, fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Stabilizer | fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT7 |
| Gene Full Name | keratin 7, type II |
| Background | Cytokeratin 7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008] |
| Function | Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). [UniProt] |
| Cellular Localization | Cytoplasm |
| Research Area | CK7/CK20 Carcinoma Study antibody |
| Calculated MW | 51 kDa |
| PTM | Arg-20 is dimethylated, probably to asymmetric dimethylarginine. |
Images (1) Click the Picture to Zoom In
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ARG62463 anti-Cytokeratin 7 antibody [OV-TL 12/30] WB image
Western blot: 30 µg of A549 cell lysate stained with ARG62463 anti-Cytokeratin 7 antibody [OV-TL 12/30] at 1:50 dilution.
Customer's Feedback
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Review for anti-Cytokeratin 7 antibody [OV-TL 12/30]
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:50
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Clone References
Serrated pathway adenocarcinomas: molecular and immunohistochemical insights into their recognition.
IHC / Human
Mammalian target of rapamycin signalling modulates amino acid uptake by regulating transporter cell surface abundance in primary human trophoblast cells.
WB / Human
Derivation and characterization of matched cell lines from primary and recurrent serous ovarian cancer.
WB / Human
Immunohistochemical comparative study of fibrosis and biliary ductular reaction in alcoholic and viral chronic hepatitis.
IHC / Human
Fusion of villous trophoblast can be visualized by localizing active caspase 8.
ICC/IF / Human
