ARG56043
anti-Cytokeratin 7 antibody [K72.7]
anti-Cytokeratin 7 antibody [K72.7] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
CK7/CK20 Carcinoma Study antibody
Overview
| Product Description | Mouse Monoclonal antibody [K72.7] recognizes Cytokeratin 7 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | K72.7 |
| Isotype | IgG1, kappa |
| Target Name | Cytokeratin 7 |
| Antigen Species | Human |
| Immunogen | A semi-purified cytokeratin preparation. |
| Conjugation | Un-conjugated |
| Alternate Names | SCL; Sarcolectin; CK-7; Cytokeratin-7; CK7; Keratin, type II cytoskeletal 7; Keratin-7; K7; K2C7; Type-II keratin Kb7 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | HeLa | ||||||||||
| Observed Size | ~ 55 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT7 |
| Gene Full Name | keratin 7, type II |
| Background | Cytokeratin 7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008] |
| Function | Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). [UniProt] |
| Cellular Localization | Cytoplasmic |
| Research Area | CK7/CK20 Carcinoma Study antibody |
| Calculated MW | 51 kDa |
| PTM | Arg-20 is dimethylated, probably to asymmetric dimethylarginine. |
Images (5) Click the Picture to Zoom In
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ARG56043 anti-Cytokeratin 7 antibody [K72.7] ICC/IF image
Immunofluorescence: Methanol-fixed HeLa cells stained with ARG56043 anti-Cytokeratin 7 antibody [K72.7] (green). Reddot (red) for nuclear staining.
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ARG56043 anti-Cytokeratin 7 antibody [K72.7] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung SCC stained with ARG56043 anti-Cytokeratin 7 antibody [K72.7].
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ARG56043 anti-Cytokeratin 7 antibody [K72.7] WB image
Western blot: HeLa cell lysate stained with ARG56043 anti-Cytokeratin 7 antibody [K72.7].
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ARG56043 anti-Cytokeratin 7 antibody [K72.7] FACS image
Flow Cytometry: PFA-fixed HeLa cells stained with ARG56043 anti-Cytokeratin 7 antibody [K72.7] (blue); Isotype control (red).
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ARG56043 anti-Cytokeratin 7 antibody [K72.7] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded ovarian carcinoma stained with ARG56043 anti-Cytokeratin 7 antibody [K72.7].
