ARG66870
anti-Cytokeratin 7 antibody
anti-Cytokeratin 7 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse
Overview
| Product Description | Mouse Monoclonal antibody recognizes Cytokeratin 7 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Target Name | Cytokeratin 7 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of Human Cytokeratin 7. |
| Conjugation | Un-conjugated |
| Alternate Names | SCL; Sarcolectin; CK-7; Cytokeratin-7; CK7; Keratin, type II cytoskeletal 7; Keratin-7; K7; K2C7; Type-II keratin Kb7 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | ~ 54 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT7 |
| Gene Full Name | keratin 7, type II |
| Background | The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008] |
| Function | Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 51 kDa |
| PTM | Arg-20 is dimethylated, probably to asymmetric dimethylarginine. [UniProt] |
Images (5) Click the Picture to Zoom In
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ARG66870 anti-Cytokeratin 7 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with anti-FoxO1 antibody (red) at 1:200 dilution, overnight at 4°C, and co-stained with ARG66870 anti-Cytokeratin 7 antibody (green) at 1:200 dilution, overnight at 4°C.
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ARG66870 anti-Cytokeratin 7 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue stained with ARG66870 anti-Cytokeratin 7 antibody (red) at 1:200 dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG66870 anti-Cytokeratin 7 antibody WB image
Western blot: HeLa, Mouse kidney and Mouse brain lysates stained with ARG66870 anti-Cytokeratin 7 antibody at 1:2000 dilution.
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ARG66870 anti-Cytokeratin 7 antibody IP image
Immunoprecipitation: 1) Input: HeLa cell lysate, 2) IP product: IP dilute 1:200. Using ARG66870 anti-Cytokeratin 7 antibody.
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ARG66870 anti-Cytokeratin 7 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue stained with ARG66870 anti-Cytokeratin 7 antibody at 1:200 dilution.
