ARG53194
anti-Cytokeratin 20 antibody
anti-Cytokeratin 20 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
CK7/CK20 Carcinoma Study antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Cytokeratin 20 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Cytokeratin 20 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to C-terminus of Human Cytokeratin 20. |
| Conjugation | Un-conjugated |
| Alternate Names | KRT21; CK20; K20; CD20; CK-20; Keratin-20; Cytokeratin-20; Keratin, type I cytoskeletal 20; Protein IT |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 30 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||
| Positive Control | Colon Carcinoma |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunogen affinity purified |
| Buffer | PBS (pH 7.6), 1% BSA and <0.1% Sodium azide |
| Preservative | <0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Cytokeratin 20 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008] |
| Function | Cytokeratin 20 plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine. [UniProt] |
| Cellular Localization | Cytoplasm |
| Research Area | CK7/CK20 Carcinoma Study antibody |
| Calculated MW | 48 kDa |
| PTM | Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity). Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. |
Images (1) Click the Picture to Zoom In
-
ARG53194 anti-Cytokeratin 20 antibody IHC-P image
Immunohistochemistry: Human Colon Adenocarcinoma stained with ARG53194 anti-Cytokeratin 20 antibody.
