ARG54051
anti-Cytokeratin 18 antibody
anti-Cytokeratin 18 antibody for ICC/IF,Western blot and Human,Monkey
Controls and Markers antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes Cytokeratin 18 |
|---|---|
| Tested Reactivity | Hu, Mk |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Target Name | Cytokeratin 18 |
| Antigen Species | Human |
| Immunogen | Purified recombinant Human Cytokeratin 18 protein fragments expressed in E. coli. |
| Conjugation | Un-conjugated |
| Alternate Names | Keratin, type I cytoskeletal 18; Cytokeratin-18; K18; CK-18; Cell proliferation-inducing gene 46 protein; Keratin-18; CYK18 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 46 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide, 0.1mg/ml BSA and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.1mg/ml BSA, 50% Glycerol |
| Concentration | 0.25 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT18 |
| Gene Full Name | keratin 18, type I |
| Background | Cytokeratin 18, together with its filament partner Cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Cytokeratin 18 involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. [UniProt] |
| Cellular Localization | Cytoplasm > perinuclear region |
| Research Area | Controls and Markers antibody; Signaling Transduction antibody |
| Calculated MW | 48 kDa |
| PTM | Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation. |
Images (2) Click the Picture to Zoom In
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ARG54051 anti-Cytokeratin 18 antibody WB image
Western blot: 20 µg of A549, HeLa and MCF7 cell lysates stained with ARG54051 anti-Cytokeratin 18 antibody at 1:2000 dilution.
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ARG54051 anti-Cytokeratin 18 antibody ICC/IF image
Immunofluorescence: HeLa cells fixed by anhydrous methanol for 2 hours at -20°C and stained with ARG54051 anti-Cytokeratin 18 antibody at 1:800 dilution.
Customer's Feedback
Excellent
Review for anti-Cytokeratin 18 antibody
Application：WB
Sample：A549, HeLa and MCF7
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:2000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
