ARG66972
anti-Cytokeratin 18 + 19 antibody
anti-Cytokeratin 18 + 19 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Cytokeratin 18 + 19 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Cytokeratin 18 + 19 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the middle region of Human Cytokeratin 18. |
| Conjugation | Un-conjugated |
| Alternate Names | Keratin, type I cytoskeletal 18; Cytokeratin-18; K18; CK-18; Cell proliferation-inducing gene 46 protein; Keratin-18; CYK18; Cytokeratin-19; K19; CK-19; K1CS; Keratin-19; CK19; Keratin, type I cytoskeletal 19 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | MDA-MB-231; Colorectal carcinoma; Breast carcinoma | ||||||||
| Observed Size | ~ 45 - 55 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300 and 20% Glycerol. |
| Preservative | 0.025% ProClin 300 |
| Stabilizer | 20% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT18 + KRT19 |
| Gene Full Name | keratin 18, type I; keratin 19, type I |
| Background | Cytokeratin 18, together with its filament partner Cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Cytokeratin 18 involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. [UniProt] |
| Highlight | Related products: Anti-Rabbit IgG secondary antibodies; Related news: Cancer Pathology Markers (SQ clones) |
| Calculated MW | 48 kDa |
| PTM | Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation. [UniProt] |
Images (4) Click the Picture to Zoom In
-
ARG66972 anti-Cytokeratin 18 + 19 antibody ICC/IF image
Immunofluorescence: MDA-MB-231 cells were fixed with 4% paraformaldehyde at RT for 10 min, permeabilized with 0.1% NP-40 at RT for 10 min and then cells were blocked with 5% BSA at RT for 30 min and stained with ARG66972 anti-Cytokeratin 18 + 19 antibody (red) at 1:150 dilution at 4°C for overnight. DAPI (blue) was used for nuclear staining.
-
ARG66972 anti-Cytokeratin 18 + 19 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG66972 anti-Cytokeratin 18 + 19 antibody at 1:100 dilution.
-
ARG66972 anti-Cytokeratin 18 + 19 antibody WB image
Western blot: A431 cell lysate stained with ARG66972 anti-Cytokeratin 18 + 19 antibody at 1:2000 dilution, overnight at 4°C.
-
ARG66972 anti-Cytokeratin 18 + 19 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colorectal carcinoma tissue stained with ARG66972 anti-Cytokeratin 18 + 19 antibody at 1:100 dilution.
