ARG55255
anti-Cytokeratin 12 antibody
anti-Cytokeratin 12 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Cytokeratin 12 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Cytokeratin 12 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 442-471 (C-terminus) of Human Cytokeratin 12. |
| Conjugation | Un-conjugated |
| Alternate Names | K12; CK-12; Cytokeratin-12; Keratin-12; Keratin, type I cytoskeletal 12 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Mouse eyeball |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT12 |
| Gene Full Name | keratin 12, type I |
| Background | KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy. [provided by RefSeq, Jul 2008] |
| Function | May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity (By similarity). [UniProt] |
| Research Area | Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 54 kDa |
Images (3) Click the Picture to Zoom In
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ARG55255 anti-Cytokeratin 12 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human colon carcinoma stained with ARG55255 anti-Cytokeratin 12 antibody.
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ARG55255 anti-Cytokeratin 12 antibody WB image
Western blot: 20 µg of Mouse eyeball lysate stained with ARG55255 anti-Cytokeratin 12 antibody.
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ARG55255 anti-Cytokeratin 12 antibody FACS image
Flow Cytometry: K562 cells stained with ARG55255 anti-Cytokeratin 12 antibody (right histogram) or isotype control antibody (left histogram). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by the primary antibody at 1:25 dilution for 60 min at 37°C, then incubated with Alexa Fluor® 488 labelled secondary antibody.
