ARG53174
anti-Cytokeratin 10 antibody
anti-Cytokeratin 10 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Signaling Transduction antibody
1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Cytokeratin 10 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Cytokeratin 10 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from C-terminus of Human Cytokeratin 10 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | KPP; K10; CK-10; BIE; Keratin, type I cytoskeletal 10; Cytokeratin-10; CK10; BCIE; Keratin-10; EHK |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Incubation Time: 30 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Skin |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunogen affinity purified |
| Buffer | PBS (pH 7.6), 1% BSA and <0.1% Sodium azide |
| Preservative | <0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Cytokeratin 10 is a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008] |
| Function | Cytokeratin 10 plays a role in the establishment of the epidermal barrier on plantar skin. (Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization. (Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur. [UniProt] |
| Cellular Localization | Cytoplasm |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 59 kDa |
Images (1) Click the Picture to Zoom In
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ARG53174 anti-Cytokeratin 10 antibody IHC-P image
Immunohistochemistry: Human Skin stained with ARG53174 anti-Cytokeratin 10 antibody.
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Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
