ARG63552
anti-Cystatin B / Stefin B antibody
anti-Cystatin B / Stefin B antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Cystatin B / Stefin B |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Cystatin B / Stefin B |
| Antigen Species | Human |
| Immunogen | C-QTNKAKHDELTYF |
| Conjugation | Un-conjugated |
| Alternate Names | Liver thiol proteinase inhibitor; EPM1; CPI-B; EPM1A; Cystatin-B; Stefin-B; PME; CST6; ULD; STFB |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 11 kDa |
Images (2) Click the Picture to Zoom In
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ARG63552 anti-Cystatin B / Stefin B antibody WB image
Western Blot: Human Lungs (35 µg protein in RIPA buffer) stained with ARG63552 anti-Cystatin B antibody at 0.1 µg/ml dilution.
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ARG63552 anti-Cystatin B / Stefin B antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63552 anti-Cystatin B / Stefin B antibody at 2.5 µg/ml dilution followed by AP-staining.
