ARG67133

anti-Cyclin B1 antibody

anti-Cyclin B1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Rat,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody.

Overview

Product Description Rabbit Polyclonal antibody recognizes Cyclin B1
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cyclin B1
Antigen Species Human
Immunogen Synthetic peptide derived from Human Cyclin B1
Conjugation Un-conjugated
Alternate Names CCNB1; Cyclin B1; CCNB; G2/Mitotic-Specific Cyclin-B1; G2/Mitotic-Specific Cyclin B1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200 - 1:1000
IHC-P1:100 - 1:300
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 58 kDa

Properties

Form Liquid
Purification Affinity chromatography purified
Buffer PBS, 0.02% Sodium azide, 0.5% BSA and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA, 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25203 Rat CCNB1

GeneID: 268697 Mouse CCNB1

GeneID: 891 Human CCNB1

Gene Symbol CCNB1
Gene Full Name cyclin B1
Background The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle. [provided by RefSeq, Aug 2017]
Function Essential for the control of the cell cycle at the G2/M (mitosis) transition. [UniProt]
Cellular Localization Cytoplasm, Cytoskeleton, Nucleus. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody.
Calculated MW 48 kDa
PTM Acetylation, Phosphoprotein, Ubl conjugation.[UniProt]

Images (6) Click the Picture to Zoom In

  • ARG67133 anti-Cyclin B1 antibody ICC/IF image

    Immunofluorescence: HeLa stained with ARG67133 anti-Cyclin B1 antibody.

  • ARG67133 anti-Cyclin B1 antibody IHC-P image

    Immunohistochemistry: Rat heart stained with ARG67133 anti-Cyclin B1 antibody at 1:200 dilution.

  • ARG67133 anti-Cyclin B1 antibody WB image

    Western blot: PC12 stained with ARG67133 anti-Cyclin B1 antibody at 1:500 dilution.

  • ARG67133 anti-Cyclin B1 antibody IHC-P image

    Immunohistochemistry: Mouse testis stained with ARG67133 anti-Cyclin B1 antibody at 1:200 dilution.

  • ARG67133 anti-Cyclin B1 antibody IHC-P image

    Immunohistochemistry: Rat testis stained with ARG67133 anti-Cyclin B1 antibody at 1:200 dilution.

  • ARG67133 anti-Cyclin B1 antibody IHC-P image

    Immunohistochemistry: Mouse kidney stained with ARG67133 anti-Cyclin B1 antibody at 1:200 dilution.