ARG43477

anti-Cyclin B1 antibody

anti-Cyclin B1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Cyclin B1.
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cyclin B1
Antigen Species Human
Immunogen Synthetic peptide derived from human Cyclin B1
Conjugation Un-conjugated
Protein Full Name G2/mitotic-specific cyclin-B1
Alternate Names CCNB

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
IP1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25203 Rat CCNB1

GeneID: 268697 Mouse CCNB1

GeneID: 891 Human CCNB1

Gene Symbol CCNB1
Gene Full Name cyclin B1
Background The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]
Function Essential for the control of the cell cycle at the G2/M (mitosis) transition. [UniProt]

Images (7) Click the Picture to Zoom In

  • ARG43477 anti-Cyclin B1 antibody IHC-P image

    Immunohistochemistry: Human lung cancer stained with ARG43477 anti-Cyclin B1 antibody at 1:100 dilution.

  • ARG43477 anti-Cyclin B1 antibody ICC/IF image

    Immunofluorescence: HeLa stained with ARG43477 anti-Cyclin B1 antibody at 1:100 dilution.

  • ARG43477 anti-Cyclin B1 antibody ICC/IF image

    Immunofluorescence: PC-12 stained with ARG43477 anti-Cyclin B1 antibody at 1:100 dilution.

  • ARG43477 anti-Cyclin B1 antibody WB image

    Western blot: C6 stained with ARG43477 anti-Cyclin B1 antibody at 1:500 dilution.

  • ARG43477 anti-Cyclin B1 antibody IHC-P image

    Immunohistochemistry: Mouse testis stained with ARG43477 anti-Cyclin B1 antibody at 1:100 dilution.

  • ARG43477 anti-Cyclin B1 antibody WB image

    Western blot: C2C12 stained with ARG43477 anti-Cyclin B1 antibody at 1:500 dilution.

  • ARG43477 anti-Cyclin B1 antibody IHC-P image

    Immunohistochemistry: Human breast cancer stained with ARG43477 anti-Cyclin B1 antibody at 1:100 dilution.