ARG59155
anti-Cullin 5 antibody
anti-Cullin 5 antibody for Western blot and Human,Mouse,Rat
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Cullin 5 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Cullin 5 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-300 of Human Cullin 5 (NP_003469.2). |
| Conjugation | Un-conjugated |
| Alternate Names | VACM-1; CUL-5; VACM1; Vasopressin-activated calcium-mobilizing receptor 1; Cullin-5 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | PC3 | ||||
| Observed Size | 98 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CUL5 |
| Gene Full Name | cullin 5 |
| Function | Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor. [UniProt] |
| Calculated MW | 91 kDa |
| PTM | Neddylated; which enhances the ubiquitination activity of SCF and prevents binding of the inhibitor CAND1. Deneddylated via its interaction with the COP9 signalosome (CSN). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG59155 anti-Cullin 5 antibody WB image
Western blot: 25 µg of PC3 cell lysate stained with ARG59155 anti-Cullin 5 antibody at 1:1000 dilution.
Specific References
Pharmacological inhibition of neddylation impairs long interspersed element 1 retrotransposition
WB / Human
