ARG62967
anti-CtBP1 antibody
anti-CtBP1 antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Microbiology and Infectious Disease antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CtBP1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IP, WB |
| Specificity | The polyclonal antibody reacts with CtBP1, a 49 kDa transcriptional corepressor, that plays a critical role in regulating gene expression. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CtBP1 |
| Antigen Species | Human |
| Immunogen | Bacterially expressed full-length human CtBP1 |
| Conjugation | Un-conjugated |
| Alternate Names | EC 1.1.1.-; CtBP1; BARS; C-terminal-binding protein 1 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Under reducing condition. ICC/IF: Staining technique: Acetone / methanol fixation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from rabbit serum by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CTBP1 |
| Gene Full Name | C-terminal binding protein 1 |
| Background | C-terminal binding protein 1 / brefeldin A (BFA)-dependent ADP-ribosylation substrate (CtBP1/BARS) is a highly conserved transcriptional corepressor, that plays a critical role in regulating gene expression patterns throughout development and in oncogenesis. CtBP regulates target gene transcription, in part, via recruitment of histone deacetylases involved in gene silencing and may act to coordonate histone modifications that regulate gene expression. |
| Function | Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Microbiology and Infectious Disease antibody |
| Calculated MW | 48 kDa |
| PTM | The level of phosphorylation appears to be regulated during the cell cycle. Phosphorylation by HIPK2 on Ser-422 induces proteasomal degradation. ADP-ribosylated; when cells are exposed to brefeldin A. Sumoylation on Lys-428 is promoted by the E3 SUMO-protein ligase CBX4. |
