ARG63803
anti-Complement factor H antibody
anti-Complement factor H antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Immune System antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Complement factor H |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, WB |
| Specificity | This antibody is expected to recognize isoform a (NP_000177.2) only. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Complement factor H |
| Antigen Species | Human |
| Immunogen | C-HLVPDRKKDQYK |
| Conjugation | Un-conjugated |
| Alternate Names | Complement factor H; ARMD4; HF1; HF2; AMBP1; ARMS1; FHL1; HUS; FH; AHUS1; H factor 1; HF; CFHL3 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||
| Positive Control | Human kidney and K562 | ||||||||
| Observed Size | ~ 140 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Oct 2011] |
| Research Area | Immune System antibody |
| Calculated MW | 139 kDa |
Images (4) Click the Picture to Zoom In
-
ARG63803 anti-Complement factor H antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63803 anti-Complement factor H antibody at 5 µg/ml dilution followed by AP-staining.
-
ARG63803 anti-Complement factor H antibody WB image
Western blot: 35 µg of Human kidney lysate (in RIPA buffer) stained with ARG63803 anti-Complement factor H antibody at 0.03 µg/ml dilution and incubated at RT for 1 hour.
-
ARG63803 anti-Complement factor H antibody WB image
Flow Cytometry: Paraformaldehyde-fixed HepG2 cells permeabilized with 0.5% Triton. Cells were stained with ARG63803 anti-Complement factor H antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa Fluor® 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
-
ARG63803 anti-Complement factor H antibody WB image
Western blot: 35 µg of K562 cell lysate (in RIPA buffer) stained with ARG63803 anti-Complement factor H antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
