ARG23545
anti-Complement C8 antibody [056B-373]
anti-Complement C8 antibody [056B-373] for ELISA,Flow cytometry,IHC-Frozen sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [056B-373] recognizes Complement C8. Mouse anti Human C8 antibody, clone 056B-373 recognizes complement component 8 (C8), a 151 kDa member of the complement C6/C7/C8/C9 family, present in serum. C8 is a terminal component of the complement system, part of both the complement membrane attack complex (MAC), and important to MAC assembly. C8 binds to the C5b-7 complex, anchored to the membrane, creating C5b-8. C5b-8 binds C9 and catalyses the polymerization of C9 molecules to form C5-b9 (MAC). C8 is thought to contain lipid binding sites, facilitating the insertion of MAC into the membrane.Defects in the alpha chain of C8 can result in complement C8 deficiency type I. Furthermore, C8 deficiencies can cause recurring bacterial infections, in particular from Neisseria meningitides. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS, IHC-Fr, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 056B-373 |
| Isotype | IgG1 |
| Target Name | Complement C8 |
| Antigen Species | Human |
| Immunogen | Purified Human Complement C8. |
| Conjugation | Un-conjugated |
| Alternate Names | C8C; Complement component C8 gamma chain |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | BBS and 0.1% Sodium azide. |
| Preservative | 0.1% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P07360 Human Complement component C8 gamma chain |
|---|---|
| Gene Symbol | C8G |
| Gene Full Name | complement component 8, gamma polypeptide |
| Background | The protein encoded by this gene belongs to the lipocalin family. It is one of the three subunits that constitutes complement component 8 (C8), which is composed of a disulfide-linked C8 alpha-gamma heterodimer and a non-covalently associated C8 beta chain. C8 participates in the formation of the membrane attack complex (MAC) on bacterial cell membranes. While subunits alpha and beta play a role in complement-mediated bacterial killing, the gamma subunit is not required for the bactericidal activity. [provided by RefSeq, Jul 2011] |
| Function | C8 is a constituent of the membrane attack complex. C8 binds to the C5B-7 complex, forming the C5B-8 complex. C5-B8 binds C9 and acts as a catalyst in the polymerization of C9. The gamma subunit seems to be able to bind retinol. [UniProt] |
| Calculated MW | 22 kDa |
