ARG66439

anti-Collagen IX alpha 3 antibody

anti-Collagen IX alpha 3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes Collagen IX alpha 3
Tested Reactivity Hu, Ms
Tested Application ICC/IF, IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Collagen IX alpha 3
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 290-370 of Human Collagen IX alpha 3.
Conjugation Un-conjugated
Alternate Names IDD; MED; EDM3; DJ885L7.4.1; Collagen alpha-3(IX) chain

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200 - 1:1000
IHC-P1:100 - 1:300
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol and 0.5% BSA
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1299 Human COL9A3

Swiss-port # Q14050 Human Collagen alpha-3(IX) chain

Gene Symbol COL9A3
Gene Full Name collagen, type IX, alpha 3
Background This gene encodes one of the three alpha chains of type IX collagen, the major collagen component of hyaline cartilage. Type IX collagen, a heterotrimeric molecule, is usually found in tissues containing type II collagen, a fibrillar collagen. Mutations in this gene are associated with multiple epiphyseal dysplasia type 3. [provided by RefSeq, Jan 2010]
Function Structural component of hyaline cartilage and vitreous of the eye. [UniProt]
Cellular Localization Secreted, extracellular space, extracellular matrix. [UniProt]
Calculated MW 64 kDa
PTM Covalently linked to the telopeptides of type II collagen by lysine-derived cross-links.

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG66439 anti-Collagen IX alpha 3 antibody ICC/IF image

    Immunofluorescence: NIH/3T3 cells stained with ARG66439 anti-Collagen IX alpha 3 antibody. The picture on the right is blocked with the synthetic peptide.

  • ARG66439 anti-Collagen IX alpha 3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human tonsil stained with ARG66439 anti-Collagen IX alpha 3 antibody. The picture on the right is blocked with the synthetic peptide.