ARG67143
anti-Collagen IV antibody
anti-Collagen IV antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Angiogenesis Study antibody; Basement Membrane Marker antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Collagen IV |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Collagen IV |
| Immunogen | Synthetic peptide |
| Conjugation | Un-conjugated |
| Alternate Names | COL4A1; Collagen Type IV Alpha 1 Chain; Collagen Alpha-1(IV) Chain; Collagen Of Basement Membrane, Alpha-1 Chain; Collagen IV, Alpha-1 Polypeptide; Collagen, Type IV, Alpha 1; COL4A1 NC1 Domain; EC 6.3.1.2; EC 3.4.23; Arresten; COL4A1s; PADMAL; BSVD1; RATOR; BSVD |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300 and 20% Glycerol. |
| Preservative | 0.025% ProClin 300 |
| Stabilizer | 20% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | COL4A1 |
| Gene Full Name | Collagen Type IV Alpha 1 Chain |
| Background | This gene encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| Function | Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. [UniProt] |
| Cellular Localization | Basement membrane, Extracellular matrix, Secreted. [UniProt] |
| Research Area | Angiogenesis Study antibody; Basement Membrane Marker antibody |
| Calculated MW | 161 kDa |
| PTM | Disulfide bond, Glycoprotein, Hydroxylation. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG67143 anti-Collagen IV antibody IHC-P image
Immunohistochemistry: Human placenta stained with ARG67143 anti-Collagen IV antibody at 1:100 dilution.
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ARG67143 anti-Collagen IV antibody WB image
Western blot: Human placenta stained with ARG67143 anti-Collagen IV antibody.
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ARG67143 anti-Collagen IV antibody IHC-P image
Immunohistochemistry: Mouse liver stained with ARG67143 anti-Collagen IV antibody at 1:100 dilution.
