ARG23197
anti-Collagen IV antibody
anti-Collagen IV antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Collagen IV Rabbit anti Human collagen IV antibodyrecogizes human collagen IV, also known as Collagen alpha-1(IV) chain. Collagen IV is a 1497 amino acid structural extracellular matrix protein and key component of the glomerular basement membrane. Arresten, comprising the c-terminal 225 amino acids of collagen IV demonstrates potent anti tumorigenic and anti angiogenic proterties (Nyberg et al. 2008). Rabbit anti Human collagen IV antibody exhibits the following reactivities with Collagen IV and related proteins:Human collagen IV100%Human types I, III and V<10%Human collagen II <1%Human plasma proteins do not interfere with collagen binding. Defects in the COL4A1 gene encoding collagen 4 can lead to the presentation of a variety of conditions including Brain small vessel disease with or without ocular anomalies (BSVD), demonstrating weakened brain blood vessels and retinal arteriolar tortuosity (Shah et al. 2010). Hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC), characterized by large renal cysts and hematuria (Plaisier et al. 2010). Porencephaly 1 (POREN1), a neurologic disease characterized by fluid filled cysts within the cerebral hemispheres, visual defect and facial paresis (Gould et al. 2005). Schizencephaly (SCHZC) presenting with full thickness cleft within the cereral hemispheres, some of the hemispheres may also be absent, replaced by cerebrospinal fluid (Yoneda et al. 2013). Other conditions resulting from mutations in the COL4A1 gene also exist presenting with some of the features described for the above conditions. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA, ICC/IF, IHC-Fr, IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Collagen IV |
| Antigen Species | Human |
| Immunogen | Collagen IV from Human placenta. |
| Conjugation | Un-conjugated |
| Alternate Names | CA44; Collagen alpha-4(IV) chain |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Pretreatment is required with 1 mg/ml pepsin in 0.5 M acetic acid for two hours at 37°C and multiple buffer washes. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, salts, Dextran and Mannitol. |
| Stabilizer | Dextran and Mannitol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | COL4A4 |
| Gene Full Name | collagen, type IV, alpha 4 |
| Background | This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. This particular collagen IV subunit, however, is only found in a subset of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and with familial benign hematuria (thin basement membrane disease). Two transcripts, differing only in their transcription start sites, have been identified for this gene and, as is common for collagen genes, multiple polyadenylation sites are found in the 3' UTR. [provided by RefSeq, Jul 2008] |
| Function | Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. [UniProt] |
| Calculated MW | 164 kDa |
| PTM | Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens. The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues. [UniProt] |
