ARG66926
anti-Collagen IV alpha 5 antibody [SQab22254]
anti-Collagen IV alpha 5 antibody [SQab22254] for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab22254] recognizes Collagen IV alpha 5 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab22254 |
| Isotype | IgG |
| Target Name | Collagen IV alpha 5 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment with COL4A5. |
| Conjugation | Un-conjugated |
| Protein Full Name | Collagen alpha-5(IV) chain |
| Alternate Names | ATS; ASLN; ATS1; CA54; Collagen alpha-5(IV) chain |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 30 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Human kidney tissue |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05%BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | COL4A5 |
| Gene Full Name | collagen type IV alpha 5 chain |
| Background | This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010] |
| Function | Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. [UniProt] |
| Cellular Localization | Basement membrane, Extracellular matrix, Secreted. [UniProt] |
| Calculated MW | 161 kDa |
| PTM | Disulfide bond, Glycoprotein, Hydroxylation |
Images (1) Click the Picture to Zoom In
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ARG66926 anti-Collagen IV alpha 5 antibody [SQab22254] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human kidney tissue stained with ARG66926 anti-Collagen IV alpha 5 antibody [SQab22254]. Antigen Retrieval: Heat tissue section in Tris-EDTA buffer (pH 9.0).
